Pollen Pollen cryopreservation can be a useful adjunct to a conservation strategy. For example, crosses might be made between disjunct populations of self-incompatible genotypes in order to produce seed. Since the longevity of some pollen is short, cryopreservation can be used to maintain viability to make crosses in plants separated by space and over different growing seasons, or it can be stored long-term to help maintain genetic diversity into the future. Expand for Selected References Ganeshan S, Rajasekharan PE, Shashikumar S, Decruze W (2008) Cryopreservation of pollen. Plant Cryopreservation – A Practical Guide pp. 443–464. doi: 10.1007/978-0-387-72276-4_17 Panella L, Wheeler L, McClintock ME (2009) Long Term Survival of Cryopreserved Sugarbeet Pollen. J Sugarbeet Res 46:1–10. doi: 10.5274/jsbr.46.1.1 Philpott M, Ballesteros D, Georgi LL, Hebard FV, Culley TM, Pence VC (2015) Seed production of American chestnut (Castanea dentata) using pollen stored 20 years in liquid nitrogen. The American Chestnut Foundation Annual Meeting, State College PA. October 2015. Rajasekharan P (2010) Pollen cryopreservation feasibility studies in Momordica doica and M. sahyadrica. IUP J Genet Evol 3:1–4. Rajasekharan PE, Ganeshan S (2019) Current perspectives on pollen cryopreservation in horticultural species. Acta Hortic 1234:47–56. doi: 10.17660/ActaHortic.2019.1234.6 Reed BM, Hummer KE (2013) Cryopreservation and maintenance of hop material in the usda germplasm collection. Acta Hortic 1010:191–198. doi: 10.17660/actahortic.2013.1010.22 Souza FVD, de Souza EH, da Silva RL (2018) Cryopreservation of pollen grains of pineapple and other bromeliads. Methods Mol Biol. 2018; 1815:279-288. doi: 10.1007/978-1-4939-8594-4_19. PMID: 29981129.