Video Resources

An unorthodox method for direct field-to-tissue culture introduction of Nuttall’s scrub oak (Quercus dumosa) by Joe Ree

Expand for More Info

I received my B.S. in BioResource Research at Oregon State University in Corvallis, OR, USA. I knew the second I stepped into the tissue culture room of the Forest Biotechnology Laboratory and saw a forest’s worth of little poplar trees lining the shelves that this was what I wanted to focus on for my career. I continued my education, first gaining my M.Sc. and then my PhD. in Plant Genetic Resources at the Federal University of Santa Catarina in Florianopolis, SC, Brazil. I studied and induced somatic embryogenesis–the asexual development of embryos from somatic tissue–in several South American palm trees during those years. I also began my first forays into cryobiology by vitrifying and regenerating somatic embryos of peach palm (Bactris gasipaes). In my current role, I will combine both tissue culture and cryobiology to develop tools to reliably cultivate, cryopreserve, and retrieve tissue from a representative population of endangered Nuttall’s scrub oak (Quercus dumosa) genotypes.

Online Resources

L.A. Withers. (1995). Collecting in vitro for genetic resources conservation. In V. Luigi Guarino, R. Rao, & R. Reid (Eds.), Collecting Plant Diversity; Technical Guidelines (Vol. 3, pp. 163–167). CAB International on behalf of the International Plant Genetic Resources Institute (IPGRI) (now Bioversity International), the Food and Agriculture Organization of the United Nations (FAO), the World Conservation Union (IUCN) and the United Nations Enviro. Retrieved from

Pence VC. (2005) In vitro collecting (IVC). I. The effect of collecting method and antimicrobial agents on contamination in temperate and tropical collections. In Vitro Cellular and Developmental Biology–Plant 41: 324-332.

Pence, V. C., & Engelmann, F. (2011). Collecting in vitro for genetic resources conservation. In L. Guarino, V. R. Rao, & E. Goldberg (Eds.), Collecting Plant Diversity; Technical Guidelines (pp. 1–5). Rome, Italy. Retrieved from

Pence, V. C., Sandoval, J. A., Villalobos, Victor, M., & Engelmann, F. (2002). In Vitro Collecting Techniques for Germplasm Conservation. In Vitro. Rome, Italy: International Plant Genetic Resources Institute. Retrieved from

Sarasan, V., Cripps, R., Ramsay, M. M., Atherton, C., McMichen, M., Prendergast, G., & Rowntree, J. K. (2006). Conservation In vitro of threatened plants—Progress in the past decade. In Vitro Cellular & Developmental Biology – Plant, 42(3), 206–214.