Video Resources

The potential of shoot tip cryopreservation as a conservation tool for oaks (Quercus spp.) by Valerie Pence

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Valerie C. Pence is the Director of Plant Research at the Center for Conservation and Research of Endangered Wildlife (CREW) at the Cincinnati Zoo & Botanical Garden. Her work focuses on applying in vitro methods and cryobiotechnologies to the conservation of exceptional species. Her research has included developing protocols for the cryo-conservation of shoot tips, embryos, gametophytes, spores, seeds, and pollen of species from across the U.S., many critically endangered.

Cryopreservation of rare and threatened Australian species shoot tips by Bryn Funnekotter

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Bryn Funnekotter is a Research Scientist at Curtin University and Kings Park Science, Biodiversity Conservation Science, DBCA, Western Australia. Bryn’s research focuses on cryopreserving rare and threatened Australian native plants, with a focus on understanding the stresses imposed by the cryopreservation process.

Cryobiotechnology tools to conserve shoot tips from exceptional species by Raquel Folgado

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Dr. Raquel Folgado serves as a cryopreservation botanist at The Huntington Botanical Gardens (Southern California, USA). She is leading a cryobiotechnology program that involves research projects on avocado and wild plants, including aloes, agaves, cacti, magnolias, cycads, and oaks. Dr. Folgado has created in vitro repositories at The Huntington to preserve and allow the distribution of Persea, Aloe, Agave, Magnolia species, and some hybrids with a commercial interest. One of her goals is to design protocols to preserve plant germplasm in liquid nitrogen to ensure the long-term conservation of threatened and rare species. Dr. Folgado’s interest extends to a better knowledge of the tolerance to abiotic constraints, which will help standardize cryopreservation methods. Methods have been optimized for many crops, but attention is needed for exceptional plant species and specialty crops like avocado. Dr. Folgado is associated with scientific societies and serves as a reviewer for several scientific journals; she collaborates with researchers worldwide to exchanging knowledge and skills in cryobiotechnology and plant abiotic stress.

Improving survival rates after cryopreservation due to genetic variation and tissue age in exceptional Hawaiian species Melicope mucronulata by Maribeth Stafford, Megan Philpott, and Valerie Pence

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Maribeth Stafford was a plant conservation research intern at the Cincinnati Zoo & Botanical Garden’s Center for Conservation Research and Endangered Wildlife (CREW) for three years. She worked with Dr. Megan Philpott to bank CREW’s collection of exceptional Hawaiian species in collaboration with the Lyon Arboretum in Honolulu. Maribeth was awarded a grant to aid her research on Melicope mucronulata by Botanic Gardens Conservation International (BGCI) in 2021. She completed her Bachelor of Science degree in Biology at the University of Cincinnati in 2021 and is currently studying to become a medical doctor.